Share this post on:

S have been correlated with the impaired liver function and regeneration, and it also implicated in both acute and chronic liver disease states [14?6]. Zn supplementation offers a protection from acute and chronic liver injury in experimental animal models [17,18], but these hepatoprotective properties have not been fully identified. In the present study, therefore, we examined the effect of Zn deficiency on diabetes-induced hepatic pathogenic damage and apoptosis as well as possible mechanisms. To this end, 1676428 we treated mice with multiple low-dose streptozotocin (MLD-STZ) to induce a type 1 diabetes. Zn deficiency was induced by chronic treatment with Zn chelator, N9N9N, N ?tetrakis (2-pyridylemethyl) AZ 876 web ethylenediamine (TPEN), as used in other studies [19,20]. After diabetic and age-matched control mice were treated with and without TPEN for four months, hepatic pathological changes and cell death along with hepatic inflammation, oxidative damage, and insulin-related signaling pathways were examined.n = 12) and age-matched control (n = 14) mice were treated intraperitoneally with TPEN (Sigma, MO, USA) at 5 mg/kg daily or with vehicle for 4 months. The selection of TPEN to chronically deplete systemic Zn is based on several previous studies that have successfully used TPEN to lower the body’s Zn levels without significant systemic toxic effects [19]. At the time of 25837696 sacrifice, the liver was harvested for histopathology and protein studies.Measurement of hepatic Zn levelsZn levels in the liver were measured by an atomic absorption spectrophotometer using air-acetylene flame after tissue was digested with nitric acid [21]. By this assay, total Zn in the tissue including free and protein-bound Zn was measured and expressed as mg/g wet tissue.Hepatic function biomarker detectionSerum plasma alanine aminotransferase (ALT) of these mice was measured using an ALT infinity enzymatic assay kit (Thermo Scientific, Waltham, MA).Histological examinationLiver tissue was fixed in 10 formalin and embedded in paraffin. Fixed liver tissues were cut into 5-mm slices. After being deparaffinized using xylene and ethanol dilutions and rehydration, tissue sections were stained with hematoxylin and eosin (H E).Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assayFor TUNEL staining, slides were stained with the reagents supplied by MedChemExpress SPDP Crosslinker ApopTag Peroxidase In Situ Apoptosis Detection Kit (Chemicon, Billerica, CA). Briefly, each slide was deparaffinized, rehydrated, and treated with proteinase K (20 mg/L) for 15 min. The endogenous peroxidase was inhibited with 3 hydrogen peroxide for 5 min, and then the slide was incubated with the TUNEL reaction mixture containing terminal deoxynucleotidyl transferase (TdT) and digoxigenin-11-dUTP for 1 h in a humidified chamber at 37uC. Then 3,3-diaminobenzidine chromogen was applied. Hematoxylin was used as counterstaining. For negative control, TdT was omitted from the reaction mixture. Apoptotic cell death was quantitatively analyzed by counting TUNEL positive cells selected randomly from ten fields at 406. Results were presented as TUNEL positive cells per 103 cells.Materials and Methods Ethics StatementThis study was carried out in the strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. The protocol was approved by the Institutional Animal Care and Use Committee of the University of Louisville (IACUC #: 10155). All.S have been correlated with the impaired liver function and regeneration, and it also implicated in both acute and chronic liver disease states [14?6]. Zn supplementation offers a protection from acute and chronic liver injury in experimental animal models [17,18], but these hepatoprotective properties have not been fully identified. In the present study, therefore, we examined the effect of Zn deficiency on diabetes-induced hepatic pathogenic damage and apoptosis as well as possible mechanisms. To this end, 1676428 we treated mice with multiple low-dose streptozotocin (MLD-STZ) to induce a type 1 diabetes. Zn deficiency was induced by chronic treatment with Zn chelator, N9N9N, N ?tetrakis (2-pyridylemethyl) ethylenediamine (TPEN), as used in other studies [19,20]. After diabetic and age-matched control mice were treated with and without TPEN for four months, hepatic pathological changes and cell death along with hepatic inflammation, oxidative damage, and insulin-related signaling pathways were examined.n = 12) and age-matched control (n = 14) mice were treated intraperitoneally with TPEN (Sigma, MO, USA) at 5 mg/kg daily or with vehicle for 4 months. The selection of TPEN to chronically deplete systemic Zn is based on several previous studies that have successfully used TPEN to lower the body’s Zn levels without significant systemic toxic effects [19]. At the time of 25837696 sacrifice, the liver was harvested for histopathology and protein studies.Measurement of hepatic Zn levelsZn levels in the liver were measured by an atomic absorption spectrophotometer using air-acetylene flame after tissue was digested with nitric acid [21]. By this assay, total Zn in the tissue including free and protein-bound Zn was measured and expressed as mg/g wet tissue.Hepatic function biomarker detectionSerum plasma alanine aminotransferase (ALT) of these mice was measured using an ALT infinity enzymatic assay kit (Thermo Scientific, Waltham, MA).Histological examinationLiver tissue was fixed in 10 formalin and embedded in paraffin. Fixed liver tissues were cut into 5-mm slices. After being deparaffinized using xylene and ethanol dilutions and rehydration, tissue sections were stained with hematoxylin and eosin (H E).Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assayFor TUNEL staining, slides were stained with the reagents supplied by ApopTag Peroxidase In Situ Apoptosis Detection Kit (Chemicon, Billerica, CA). Briefly, each slide was deparaffinized, rehydrated, and treated with proteinase K (20 mg/L) for 15 min. The endogenous peroxidase was inhibited with 3 hydrogen peroxide for 5 min, and then the slide was incubated with the TUNEL reaction mixture containing terminal deoxynucleotidyl transferase (TdT) and digoxigenin-11-dUTP for 1 h in a humidified chamber at 37uC. Then 3,3-diaminobenzidine chromogen was applied. Hematoxylin was used as counterstaining. For negative control, TdT was omitted from the reaction mixture. Apoptotic cell death was quantitatively analyzed by counting TUNEL positive cells selected randomly from ten fields at 406. Results were presented as TUNEL positive cells per 103 cells.Materials and Methods Ethics StatementThis study was carried out in the strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. The protocol was approved by the Institutional Animal Care and Use Committee of the University of Louisville (IACUC #: 10155). All.

Share this post on:

Author: email exporter