Infection Positive Negative Lauren’s classification Intestinal-type Diffuse-type Lymph node involvement Positive NegativenNo. of positive stains (n, )No. of negative stains (n, )P value20 21 224(20.0) 11(52.3) 13(59.1) 20(66.7)*16(80.0) 10(47.7) 9(40.9) 10(33.3)0.2416(66.7) 32(46.4)8(33.3) 37(53.6)0.3925(64.1) 8(63.6)14(35.9) 5(36.4)0.2720(74.1) 13(52.0)7(25.9) 12(48.0)0.Data were analyzed by Chi-Square tests. *, significantly different from controls (P,0.05). GC: gastric cancer; Hp:Helicobacter pylori. doi:10.1371/journal.pone.0054249.tTGF-b Roles in Tumor-Cell Interaction with PBMCsTGF-b Roles in Tumor-Cell Interaction with PBMCsFigure 2. TGF-b1 and Peptide M web TGF-b2 mRNA profiles in gastric precancer and carcinoma. (A) TGF-b1 mRNA levels in the sequence from controls (n = 20), precancer (PC) (n = 21), early gastric cancer (EGC) (n = 22), to advanced gastric cancer (AGC) (n = 30). Data are given as means 6 SD of transcript levels normalized to GAPDH. (B) Corresponding TGF-b2 mRNA levels in the same sequence. (C) and (D) TGF-b1 levels were upregulated and TGF-b2 levels were downregulated in tumor tissues, compared to peritumoral tissues from the same patients. Levels were normalized to GAPDH. Data from qRT-PCR in 20 paired cases are shown. (E) and (F) Significant positive correlations between TGF-b1 and Smad2/Smad7, using a bivariate correlation model. Data represent the transcript levels in 36 cases of GC after normalization to GAPDH. (G)Serum concentrations of TGF-b1 and TGFb2 measured by ELISA were significantly higher in early and advanced GC compared to controls (F = 4.745 and P = 0.018; F = 4.939 and P = 0.015, respectively). There was no significant difference between early and advanced GC. Ctrl: controls volunteers; EGC: early gastric cancer; AGC: advanced gastric cancer. doi:10.1371/journal.pone.0054249.gthere were no significant difference in the results of TGF-b1 and TGF-b2 mRNA levels in GC cells in direct coculture model using PBMCs isolated from GC patients or controls (Figure 3A), and these data were therefore pooled for analysis. Furthermore, concentrations of TGF-b1 in the cell supernatant of cocultures were significantly buy MNS increased compared to those in PBMCs or GCs cultured alone in a FBS-free environment (P,0.05) and its levels in the direct coculture group were significantly higher than those in the indirect group (P = 0.029); however, although TGF-b2 levels were also increased in direct cocultures, the differences after cocultures were not significant (Figure 3B). We subsequently investigated the effect of serum on the interaction between tumor cells and PBMCs. Surprisingly, TGFb1 and TGF-b2 concentrations in the indirect group, compared to that in the FBS-free condition, were inversely 23977191 higher than those in the direct group after the addition of FBS. Moreover, the concentrations of TGF-b1 and TGF-b2 in the cell supernatant were significantly increased in indirect groups (P,0.05), but they were only slightly increased in direct groups (P.0.05), by the addition of FBS (Figure 3C). This suggests that an enriched environment may facilitate cytokine production in indirect not in direct communication. Further, to determine the origins of the cytokines, TGF-b1 and TGF-b2 mRNA levels were measured in GC cells and PBMCs respectively. Compared to monoculture, TGF-b1 mRNA levels were increased approximately 3-fold in the direct group and 2-fold in the indirect group in GC cells after coculture with PBMCs; TGF-b2 mRNA levels were signif.Infection Positive Negative Lauren’s classification Intestinal-type Diffuse-type Lymph node involvement Positive NegativenNo. of positive stains (n, )No. of negative stains (n, )P value20 21 224(20.0) 11(52.3) 13(59.1) 20(66.7)*16(80.0) 10(47.7) 9(40.9) 10(33.3)0.2416(66.7) 32(46.4)8(33.3) 37(53.6)0.3925(64.1) 8(63.6)14(35.9) 5(36.4)0.2720(74.1) 13(52.0)7(25.9) 12(48.0)0.Data were analyzed by Chi-Square tests. *, significantly different from controls (P,0.05). GC: gastric cancer; Hp:Helicobacter pylori. doi:10.1371/journal.pone.0054249.tTGF-b Roles in Tumor-Cell Interaction with PBMCsTGF-b Roles in Tumor-Cell Interaction with PBMCsFigure 2. TGF-b1 and TGF-b2 mRNA profiles in gastric precancer and carcinoma. (A) TGF-b1 mRNA levels in the sequence from controls (n = 20), precancer (PC) (n = 21), early gastric cancer (EGC) (n = 22), to advanced gastric cancer (AGC) (n = 30). Data are given as means 6 SD of transcript levels normalized to GAPDH. (B) Corresponding TGF-b2 mRNA levels in the same sequence. (C) and (D) TGF-b1 levels were upregulated and TGF-b2 levels were downregulated in tumor tissues, compared to peritumoral tissues from the same patients. Levels were normalized to GAPDH. Data from qRT-PCR in 20 paired cases are shown. (E) and (F) Significant positive correlations between TGF-b1 and Smad2/Smad7, using a bivariate correlation model. Data represent the transcript levels in 36 cases of GC after normalization to GAPDH. (G)Serum concentrations of TGF-b1 and TGFb2 measured by ELISA were significantly higher in early and advanced GC compared to controls (F = 4.745 and P = 0.018; F = 4.939 and P = 0.015, respectively). There was no significant difference between early and advanced GC. Ctrl: controls volunteers; EGC: early gastric cancer; AGC: advanced gastric cancer. doi:10.1371/journal.pone.0054249.gthere were no significant difference in the results of TGF-b1 and TGF-b2 mRNA levels in GC cells in direct coculture model using PBMCs isolated from GC patients or controls (Figure 3A), and these data were therefore pooled for analysis. Furthermore, concentrations of TGF-b1 in the cell supernatant of cocultures were significantly increased compared to those in PBMCs or GCs cultured alone in a FBS-free environment (P,0.05) and its levels in the direct coculture group were significantly higher than those in the indirect group (P = 0.029); however, although TGF-b2 levels were also increased in direct cocultures, the differences after cocultures were not significant (Figure 3B). We subsequently investigated the effect of serum on the interaction between tumor cells and PBMCs. Surprisingly, TGFb1 and TGF-b2 concentrations in the indirect group, compared to that in the FBS-free condition, were inversely 23977191 higher than those in the direct group after the addition of FBS. Moreover, the concentrations of TGF-b1 and TGF-b2 in the cell supernatant were significantly increased in indirect groups (P,0.05), but they were only slightly increased in direct groups (P.0.05), by the addition of FBS (Figure 3C). This suggests that an enriched environment may facilitate cytokine production in indirect not in direct communication. Further, to determine the origins of the cytokines, TGF-b1 and TGF-b2 mRNA levels were measured in GC cells and PBMCs respectively. Compared to monoculture, TGF-b1 mRNA levels were increased approximately 3-fold in the direct group and 2-fold in the indirect group in GC cells after coculture with PBMCs; TGF-b2 mRNA levels were signif.
