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And two were clade B. At that point, however, the potency of neutralization was weak and the breadth of neutralization wasCo-Evolving bNAbs during HIV-InfectionFigure 6. Timeline of the epitope evolution of Nafarelin web cross-reactive NAb responses in AC053. The breadth of neutralizing antibody responses (i.e., the percent of heterologous isolates neutralized by plasma samples out of the total isolates tested [14]), was plotted for all available time-points for subject AC053. The arrows on the timeline correspond to approximate years post infection when particular neutralizing antibody specificities became evident. Breadth is colorcoded as follows: blue 0?9 , green 20?9 , orange 40?4 , red 75?100 . doi:10.1371/journal.pone.0049610.gnarrow. In addition, several isolates that are susceptible to PG9 were resistant to neutralization by 11967625 this plasma. Overall, these observations suggested to us that, at its earliest development, the glycan-dependent neutralizing activity in AC053 plasma was not due to PG9-like antibodies. Of course, one could also argue that PG9-like antibodies began emerging at that point of infection, but that their VH and VL antibody domains had not yet incurred Arg8-vasopressin site somatic mutations that are required for the broad neutralizing ability of PG9. In the absence of longitudinally isolated MAbs from AC053 it is not possible to address this point directly. Broader cross-neutralizing antibody responses capable of neutralizing at least 50 of isolates tested (from clades A, B and C) became first apparent at approximately 3 ypi and were due to anti-CD4-BS neutralizing antibodies (Figure 6 and [14]). As we extensively discussed previously, these anti-CD4-BS cross-neutralizing activities were not effective against all isolates that were susceptible to neutralization by the AC053 plasma [14]. For example, they were not effective against the CAAN or TRO.11 viruses. Even the anti-CD4-BS neutralizing activities of plasmas isolated later in infection, which were broader and more potent, were ineffective against these and other viruses. At 3 ypi, crossneutralizing specificities that are dependent on the presence of a glycan at position 160 were not evident in AC053. This second cross-neutralizing specificity became apparent sometime around4.30 ypi. Because of its dependency on the 160 glycan but not on glycans positioned in regions of Env targeted by the PGT-like antibodies or 2G12-like antibodies, we believe that this second cross-neutralizing specificity is due to PG9-like antibodies. We do not believe it is due to PG16-like antibodies, because the neutralizing activity 1407003 of PG16 cannot be blocked by SF162K160N gp120, while that of PG9 and of the AC053 plasma antibodies are efficiently blocked by that recombinant protein. We used two independent methods to demonstrate the presence of a PG9-like glycan-dependent epitope specificity of the broadly neutralizing antibody response in AC053. The use of glycosidase inhibitors, such as kifunensine, to enrich high mannose glycans is a well-established method and has been previously used to identify glycan-dependent epitopes targeted by anti-HIV antibody responses [26,29,51]. Of note, the nature of the glycosylation pattern on HIV Env can be influenced by the host cell and culture conditions used [60,61]. The majority of studies on antibody responses to HIV have used pseudoviruses produced in cell lines, such as the 293T used in this study. However, it is possible that these viruses have different N-linked glycosylat.And two were clade B. At that point, however, the potency of neutralization was weak and the breadth of neutralization wasCo-Evolving bNAbs during HIV-InfectionFigure 6. Timeline of the epitope evolution of cross-reactive NAb responses in AC053. The breadth of neutralizing antibody responses (i.e., the percent of heterologous isolates neutralized by plasma samples out of the total isolates tested [14]), was plotted for all available time-points for subject AC053. The arrows on the timeline correspond to approximate years post infection when particular neutralizing antibody specificities became evident. Breadth is colorcoded as follows: blue 0?9 , green 20?9 , orange 40?4 , red 75?100 . doi:10.1371/journal.pone.0049610.gnarrow. In addition, several isolates that are susceptible to PG9 were resistant to neutralization by 11967625 this plasma. Overall, these observations suggested to us that, at its earliest development, the glycan-dependent neutralizing activity in AC053 plasma was not due to PG9-like antibodies. Of course, one could also argue that PG9-like antibodies began emerging at that point of infection, but that their VH and VL antibody domains had not yet incurred somatic mutations that are required for the broad neutralizing ability of PG9. In the absence of longitudinally isolated MAbs from AC053 it is not possible to address this point directly. Broader cross-neutralizing antibody responses capable of neutralizing at least 50 of isolates tested (from clades A, B and C) became first apparent at approximately 3 ypi and were due to anti-CD4-BS neutralizing antibodies (Figure 6 and [14]). As we extensively discussed previously, these anti-CD4-BS cross-neutralizing activities were not effective against all isolates that were susceptible to neutralization by the AC053 plasma [14]. For example, they were not effective against the CAAN or TRO.11 viruses. Even the anti-CD4-BS neutralizing activities of plasmas isolated later in infection, which were broader and more potent, were ineffective against these and other viruses. At 3 ypi, crossneutralizing specificities that are dependent on the presence of a glycan at position 160 were not evident in AC053. This second cross-neutralizing specificity became apparent sometime around4.30 ypi. Because of its dependency on the 160 glycan but not on glycans positioned in regions of Env targeted by the PGT-like antibodies or 2G12-like antibodies, we believe that this second cross-neutralizing specificity is due to PG9-like antibodies. We do not believe it is due to PG16-like antibodies, because the neutralizing activity 1407003 of PG16 cannot be blocked by SF162K160N gp120, while that of PG9 and of the AC053 plasma antibodies are efficiently blocked by that recombinant protein. We used two independent methods to demonstrate the presence of a PG9-like glycan-dependent epitope specificity of the broadly neutralizing antibody response in AC053. The use of glycosidase inhibitors, such as kifunensine, to enrich high mannose glycans is a well-established method and has been previously used to identify glycan-dependent epitopes targeted by anti-HIV antibody responses [26,29,51]. Of note, the nature of the glycosylation pattern on HIV Env can be influenced by the host cell and culture conditions used [60,61]. The majority of studies on antibody responses to HIV have used pseudoviruses produced in cell lines, such as the 293T used in this study. However, it is possible that these viruses have different N-linked glycosylat.

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