In human (a) and mouse (b) at p-value of 0.01. Marked in red and blue in the top line are the maternally and paternally expressed genes, respectively. (TIFF)Author ContributionsConceived and designed the experiments: MH SI MP VH. Performed the experiments: MH. Analyzed the data: MH SI MP VH. Wrote the paper: MH MP VH.
Heart failure (HF) is a global health problem, associated with poor clinical outcomes and substantial economic burden to the healthcare system [1,2]. Approximately, 23 million people worldwide are living with HF [2]. The population estimates of HF prevalence ranges between 2 and 10 , with a higher prevalence in the elderly [3]. Plasma/serum concentrations of natriuretic peptides, N-terminal proB-type natriuretic peptide (NT-proBNP, 76 AA) or B-type natriuretic peptide (BNP, 32 AA) are currently used to diagnose HF [4?]. Several companies including Roche Diagnostics commercialise NT-proBNP immunoassays targeting various fragments of the NT-proBNP molecule (middle part of the NTproBNP molecule is glycosylated). Therefore, the NT-proBNP results are not comparable across laboratories [8?0]. Currentblood-based `sandwich’ immunoassays use monoclonal and polyclonal antibodies targeting different epitopes to quantify plasma levels of NT-proBNP and BNP [11?4]. This may complicate interpretation of plasma levels of NT-proBNP/BNP for diagnosing and monitoring HF, especially if a patient accesses different laboratory services that use different assays/platforms. These differences 1379592 will only be minimised with improved understanding of the molecular forms and glycosylation patterns of NTproBNP and BNP in the circulation. Human saliva composition reflects our body’s health and well being and about 20 of proteins that are present in the blood are also found in saliva [15], which highlights the diagnostic potential of saliva. Saliva does not clot like blood, and its collection is noninvasive [16?8]. Saliva samples are relatively easy to handle in comparison to blood collection and processing thereby decreasing the risk of contracting blood-borne infectious organisms [19?1].Relevance of Salivary NT-ProBNP and Heart FailureFurthermore, avoiding the need for a phlebotomist enables multiple saliva sample collections within a day by unskilled people. The half-life of BNP is approximately 20 minutes and that of NT-proBNP is around 60?0 minutes [22,23]. Hence, NTproBNP clearance from blood is slower than its counterpart BNP, allowing CPI-455 custom synthesis possible movement of the former molecule into the saliva through various routes, but mainly via the gingival crevicular fluid [24]. We hypothesise that the relatively long half-life of NT-proBNP in circulation enables substantial movement of NT-proBNP from blood into saliva. The aims of our study were to develop an immunoassay to detect NTproBNP in saliva and to determine if there is a correlation with plasma levels.2.2 SamplesBlood samples were CYT387 collected into EDTA tubes (Greiner VACUETTEH # 454023, Greiner Bio-one, Graz, Austria) and then immediately centrifuged at 30006g at RT for 10 minutes. The plasma samples were divided into aliquots, and stored at 280uC until analysed. Saliva samples were collected in sterile urine containers (Sarstedt, Australia) and stored at 280uC until analysed. For salivary protein analysis, unstimulated saliva is the preferred method [18,21]. Unstimulated resting saliva was collected by the draining or drooling method described by Navazesh and Christensen [25,26]. Volunteers were asked t.In human (a) and mouse (b) at p-value of 0.01. Marked in red and blue in the top line are the maternally and paternally expressed genes, respectively. (TIFF)Author ContributionsConceived and designed the experiments: MH SI MP VH. Performed the experiments: MH. Analyzed the data: MH SI MP VH. Wrote the paper: MH MP VH.
Heart failure (HF) is a global health problem, associated with poor clinical outcomes and substantial economic burden to the healthcare system [1,2]. Approximately, 23 million people worldwide are living with HF [2]. The population estimates of HF prevalence ranges between 2 and 10 , with a higher prevalence in the elderly [3]. Plasma/serum concentrations of natriuretic peptides, N-terminal proB-type natriuretic peptide (NT-proBNP, 76 AA) or B-type natriuretic peptide (BNP, 32 AA) are currently used to diagnose HF [4?]. Several companies including Roche Diagnostics commercialise NT-proBNP immunoassays targeting various fragments of the NT-proBNP molecule (middle part of the NTproBNP molecule is glycosylated). Therefore, the NT-proBNP results are not comparable across laboratories [8?0]. Currentblood-based `sandwich’ immunoassays use monoclonal and polyclonal antibodies targeting different epitopes to quantify plasma levels of NT-proBNP and BNP [11?4]. This may complicate interpretation of plasma levels of NT-proBNP/BNP for diagnosing and monitoring HF, especially if a patient accesses different laboratory services that use different assays/platforms. These differences 1379592 will only be minimised with improved understanding of the molecular forms and glycosylation patterns of NTproBNP and BNP in the circulation. Human saliva composition reflects our body’s health and well being and about 20 of proteins that are present in the blood are also found in saliva [15], which highlights the diagnostic potential of saliva. Saliva does not clot like blood, and its collection is noninvasive [16?8]. Saliva samples are relatively easy to handle in comparison to blood collection and processing thereby decreasing the risk of contracting blood-borne infectious organisms [19?1].Relevance of Salivary NT-ProBNP and Heart FailureFurthermore, avoiding the need for a phlebotomist enables multiple saliva sample collections within a day by unskilled people. The half-life of BNP is approximately 20 minutes and that of NT-proBNP is around 60?0 minutes [22,23]. Hence, NTproBNP clearance from blood is slower than its counterpart BNP, allowing possible movement of the former molecule into the saliva through various routes, but mainly via the gingival crevicular fluid [24]. We hypothesise that the relatively long half-life of NT-proBNP in circulation enables substantial movement of NT-proBNP from blood into saliva. The aims of our study were to develop an immunoassay to detect NTproBNP in saliva and to determine if there is a correlation with plasma levels.2.2 SamplesBlood samples were collected into EDTA tubes (Greiner VACUETTEH # 454023, Greiner Bio-one, Graz, Austria) and then immediately centrifuged at 30006g at RT for 10 minutes. The plasma samples were divided into aliquots, and stored at 280uC until analysed. Saliva samples were collected in sterile urine containers (Sarstedt, Australia) and stored at 280uC until analysed. For salivary protein analysis, unstimulated saliva is the preferred method [18,21]. Unstimulated resting saliva was collected by the draining or drooling method described by Navazesh and Christensen [25,26]. Volunteers were asked t.
