Recombinant Mouse IFN alpha/beta R1 Protein,His-Avi Tag

Description :
Synonyms AVP; IFNAR; IFN-alpha-REC; IFN-aR1 Source Mouse IFN alpha/beta R1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu27-Thr429.[Accession | P33896] Molecular Weight The protein has a predicted MW of 46.9 kDa. Due to glycosylation, the protein migrates to 67-70 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity SPR Data: Mouse IFNAR1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IFN alpha 1, hFc Tag with an affinity constant of 3.61 nM as determined in SPR assay . Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.