Recombinant Human IL-5 R alpha/CD125 Protein,His-Avi Tag

Description :
Synonyms CD125; IL-5 R alpha; HSIL5R3; IL5R Source Human IL-5 R alpha/CD125 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Asp21-Glu335.[Accession | Q01344-1] Molecular Weight The protein has a predicted MW of 38.7 kDa. Due to glycosylation, the protein migrates to 50-68 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity SPR Data: Human IL-5, His Tag immobilized on CM5 Chip can bind Human IL-5 R alpha, His Tag with an affinity constant of 4.9nM as determined in a SPR assay . Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.