Recombinant Human IL-12B/p40/NKSF2 Protein,His Tag

Description :
Synonyms IL-12 subunit p40; CLMF p40;IL-12B; CLMF Source Human IL-12B/p40/NKSF2 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ile23-Ser328.[Accession | P29460] Molecular Weight The protein has a predicted MW of 34.7 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Human IL-12B, His Tag at 1g/ml (100l/Well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 18.5ng/ml determined by ELISA .Immobilized Human IL-12B, His Tag at 1g/ml (100l/well) on the plate. Dose response curve for Human IL-12 R beta 1, hFc Tag with the EC50 of 0.26g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.