Recombinant Cynomolgus PCSK9 Protein,His Tag

Description :
Synonyms PCSK9; Source Cynomolgus PCSK9 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Glu151-Gln811.[Accession | A0A2K5TZC4] Molecular Weight Due to autocatalytic cleavage, the protein release the pro-form (59 kDa) and mature form (14 kDa). Due to glycosylation, the protein migrates to 65-70 kDa (pro-form) and 15-20 kDa (mature form) based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Cynomolgus PCSK9, His Tag at 5g/ml (100l/Well) on the plate. Dose response curve Anti-PCSK9 Antibody, hFc Tag with the EC50 of 81.5ng/ml determined by ELISA . Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.