Recombinant Human LCAT Protein,His Tag

Description :
Synonyms LCAT;PAF acetylhydrolase; Source Human LCAT Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Phe25-Glu440.[Accession | P04180] Molecular Weight The protein has a predicted MW of 48.2 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Human LCAT, His Tag at 1g/ml (100l/Well) on the plate. Dose response curve for Anti-LCAT Antibody, hFc Tag with the EC50 of 13.0ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.