Recombinant Human GM-CSF R alpha Protein,hFc Tag

Description :
Synonyms GMR-alpha; GMCSFR-alpha;GM-CSF-R-alpha; CSF2RY Source Human GM-CSF R alpha Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Glu23-Gly320.[Accession | P15509-1] Molecular Weight The protein has a predicted MW of 63.5 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human GM-CSF at 2g/ml (100l/Well) on the plate. Dose response curve for Human GM-CSF R alpha, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA . Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.