Recombinant Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein,His-Avi Tag

Description :
Synonyms ITGAV&ITGB6;Integrin alpha V beta 6;V6 Source Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Phe31-Val992(ITGAV) acidic tail & Gly22-Asn707(ITGB6) basic tail.[Accession | P06756-1(ITGAV)&P18564-1(ITGB6)] Molecular Weight The protein has a predicted MW of 114 kDa (ITGAV)&79.1 kDa (ITGB6). Due to glycosylation, the protein migrates to 90-150 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human ITGAV&ITGB6, His Tag at 1g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Tag with the EC50 of 0.49g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.