Recombinant Mouse IL-2 R alpha/CD25 Protein,His Tag

Description :
Synonyms CD25; IDDM10; IL-2 R alpha; IL2R; IL2RA; p55; TCGFR Source Mouse IL-2 R alpha/CD25 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Glu22-Lys236.[Accession | P01590] Molecular Weight The protein has a predicted MW of 25.76 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Mouse IL-2 R alpha, His Tag at 2g/ml (100l/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC50 of 65.2ng/ml determined by ELISA . Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.