Recombinant Cynomolgus IL-5 Protein,His Tag

Description :
Synonyms Interleukin-5; IL-5; T-cell replacing factor; TRF; EDF Source Cynomolgus IL-5 Protein is expressed from HEK293 with His tag at the N-Terminus. It contains Ile20-Ser134.[Accession | A0A2K5U1E7] Molecular Weight The protein has a predicted MW of 14.25 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Cynomolgus IL-5, His Tag at 1g/ml (100l/well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 66.6ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.