Recombinant Cynomolgus TNF alpha Protein,His Tag

Description :
Synonyms APC1 protein; Cachectin; DIF; TNF; TNFalpha; TNFATNF; TNFSF1A; TNFSF2; TNFA; TNF; DIF; TNFSF2;TNF-alpha Source Cynomolgus TNF alpha Protein is expressed from HEK293 with His tag at the N-Terminus. It contains Val77-Leu233.[Accession | P79337] Molecular Weight The protein has a predicted MW of 18.35 kDa. Due to glycosylation, the protein migrates to 19-25 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Cynomolgus TNF alpha, His Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Human TNFR1, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA . Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.