Recombinant Human GDF-7/BMP-12

Description :
FAQ COA 已发表文献 Growth Differentiation Factor-7 (GDF-7; also called BMP-12 and CDMP-3) is a member of the BMP family of TGF-beta superfamily proteins. GDF7 is synthesized as a large precursor protein that consists of an N-terminal 19 amino acid (aa) signal sequence, a 302 aa pro region and a 129 aa C-terminal mature peptide. At the amino acid level, mature human GDF-7 shares 85% and 88% aa sequence identity with mature GDF-7 in mouse and rat, respectively. Mature human GDF-7 lacks a glycine repeat that is found in both mouse and rat GDF-7. Based on sequence similarity, GDF-7 is categorized with GDF-5 and -6, as a subgroup within the BMP family. GDF-7 functions as a homodimer and elicits its bioactivity by mediating the formation of a heterodimeric receptor complex consisting of a type 1 (BMPR-IB) and a type II (BMPR-II or Activin RII) serine/threonine kinase receptor. GDF-7 signaling results in the phosphorylation and activation of cytosolic Smad proteins (Smad1, 5, and 8). Product Properties Synonyms BMP12GDF7 Accession Q7Z4P5 GeneID 151449 Source E.coli-derived Human GDF-7/BMP-12, Thr322-Arg450. Molecular Weight Approximately 28.0 kDa. AA Sequence TALAGTRTAQ GSGGGAGRGH GRRGRSRCSR KPLHVDFKEL GWDDWIIAPL DYEAYHCEGL CDFPLRSHLE PTNHAIIQTL LNSMAPDAAP ASCCVPARLS PISILYIDAA NNVVYKQYED MVVEACGCR Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% by SDS-PAGE and HPLC analyses. Biological Activity The ED50as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 1.0 g/mL, corresponding to a specific activity of > 1000 IU/mg. Fully biologically active when compared to standard. Endotoxin Formulation Lyophilized from a 0.2 m filtered concentrated solution in 30% Acetonitrile and 0.1% TFA. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.