Recombinant Human IL-17R alpha/CD217 Protein,His Tag

Description :
Source Human IL-17R alpha/CD217 Protein is expressed from HEK293 with His tag at the C-terminal. It contains Leu33-Trp320.[Accession |Q96F46-1] Molecular Weight The protein has a predicted MW of 34.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per ug by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity ELISA Data: Immobilized Human IL-17R alpha, His Tag at 2g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human IL-17A, His Tag with the EC50 of 0.15g/ml determined by ELISA SPR Data:Human IL-17R alpha, His Tag immobilized on CM5 Chip can bind Human IL-17A, His Tag with an affinity constant of 1.57 nM as determined in SPR assay (Biacore T200). Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.