Product Name :
Human HGF Protein
EN
Hepatopoietin-A; SF; HPTA; HGF; DFNB39; F-TCF; F-TCFB; HGFB
Description:
| Human HGF Protein with the C-His Tag |Source: The reco|>95% by SEC-HPLC and SDS-|Less than 1 EU per ug of protein as determined by LAL method. |Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |Reconstitution: It is recommended to reconstitute the lyophilized reco|The reco|>95% by SEC-HPLC and SDS-|Less than 1 EU per ug of protein as determined by LAL method. |Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |Reconstitution: It is recommended to reconstitute the lyophilized reco|The recombinant Human HGF Protein is shipped with ice |Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution.
Source:
The recombinant Human HGF Protein, (amino acids Gln32-Ser728) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight :
The HGF single chain can be processed into the active form of disulfide-linked heterodimer of α and β chain.The recombinant Human HGF protein with the His tag at the C-terminus has a predicted MW of 79.6 kDa (after cleavage of the signal peptide). Due to glycosylation, the protein migrates to 60-65 kDa and 30-40 kDa corresponding to α chain and β chain based on Bis-Tris PAGE result.
Activity:
Immobilized recombinant Human HGF protein with the C-His tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human HGF R protein with the C-hFc tag with the EC50 of 9.9 ng/ml determined by ELISA.
Purity :
>95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin:
LevelLess than 1 EU per ug of protein as determined by LAL method.
Formulation :
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution:
It is recommended to reconstitute the lyophilized recombinant Human HGF Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. BP004702Human HGF Protein with the C-hFc Tag SourceThe recombinant Human HGF Protein, (amino acids Gln32-Ser728) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Shipping:
The recombinant Human HGF Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage :
/strong>Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
283173-50-2 Molecular Weight 79517-01-4 Description PMID:27198494 MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com
