Daily Archives: January 16, 2017

First, a given PH domain was added to target membranes containing PIP3 and PS to form the membrane-bound complex, then the competitive inhibitor IP6

1st, a provided PH domain was added to concentrate on membranes that contains PIP3 and PS to form the membrane-sure complex, then the competitive inhibitor IP6 (inositol-hexaphosphate) was titrated into the sample to displace the PH domain from its target membrane. Displacement was monitored by an recognized protein-to-membrane FRET assay (Determine 2), yielding an equilibrium…

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