Daily Archives: March 2, 2017

The input proteins were subjected to Western blotting with anti-His and anti-MBP antibodies, respectively, as shown in middle and bottom

Figure one. Expression of recombinant WhNV AMI-1 protein A. (A) Schematic representation of the expression technique of WhNV protein A. Protein A ORF was cloned into pMAL-c2X and expressed as C-terminal fusion proteins with MBP (MBP-protA). To manipulate the vector, we mutated a BamH I restriction endonuclease web site on protein A ORF sequences. (B)…

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