Daily Archives: March 3, 2017

Data represented here are from one of three independent experiments, all of which yielded similar results infection was associated with significantly augmented histone phosphorylation and acetylation

Following forty five min of incubation, ChIP assays ended up conducted as described in Supplies and Approaches. Immunoprecipitations have been performed utilizing Abs specific to phosphorylated H3 (IP phospho-H3) (I) or acetylated H3 (IP acetyl-H3) (J), and traditional RT-PCR was done utilizing primers specific to the IL-ten promoter. Data represented below are from one of…

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