Recombinant Human GITR/TNFRSF18(His Tag)

Description :
FAQ COA 已发表文献 GITRLigand, also known as TNFSF18 and TL6, is an approximately 30kDa typeII transmembrane glycoprotein in the TNF superfamily. Human GITR Ligand consists of a 50amino acid (aa) cytoplasmic domain, a 21aa transmembrane segment, and a 128aa extracellular domain (ECD) . Within the ECD, human GITR Ligand shares 56%and 60%aa sequence identity with mouse and rat GITRLigand, respectively. GITR Ligand is expressed on antigen presenting cells, 产品性质 别名Synonyms CD357; GITR; GITR-D; TNFRSF18; AITR Uniprot No. Q9Y5U5-1 Source Recombinant Human GITR/TNFRSF18 Protein is expressed from HEK293 Cellswith His tag at the C-terminal. It contains Gln26-Glu161. 分子量(Molecular Weight) The protein has a predicted MW of 15.4 kDa. Due to glycosylation, the protein migrates to 25-28 kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity 1.ELISA DataImmobilized Human GITR, His Tag at 1g/ml (100l/Well). Dose response curve for Biotinylated Human GITR Ligand Trimer, His Tag with the EC50 of 0.2g/ml determined by ELISA. 2.ELISA DataImmobilized Human GITR, His Tag at 0.5g (100l/Well) on the plate. Dose response curve for Anti-GITR Antibody, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA. 3.SPR DataHuman GITR, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human GITR Ligand Trimer, hFc Tag with an affinity constant of 4.76 nM as determined in SPR assay . 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mLis recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.