Biotinylated Recombinant Human CD40/TNFRSF5(His-Avi Tag)

Description :
FAQ COA 已发表文献 CD40, also known as TNFRSF5, is a 45-50 kDa type I transmembrane glycoprotein member of the TNF receptor superfamily. Mature human CD40 consists of a 173 amino acid (aa) extracellular domain, a transmembrane domain, and a 62 aa cytoplasmic domain. CD40 serves multiple functions in both hematopoietic and epithelial cancers and is a target for tumor immunotherapy. Dysregulation of CD40/CD40 Ligand expression and interactions contributes to the immune deficiency associated with HIV infection and AIDS. It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis. 产品性质 别名 CD40; CD40L receptor; TNFRSF5; CD40 antigen; CD40 molecule; CDw40; MGC9013; Uniprot No. P25942 Recombinant Biotinylated Human CD40/TNFRSF5Protein is expressed from HEK293Cellswith His tag and Avi tag at the C-terminal. It contains Glu21-Arg193. 分子量 Approximately 22.1 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. ELISAData:Immobilized Biotinylated Human CD40, His Tag at 1g/ml (100l/Well) on the plate. Dose response curve for Human CD40L, hFc Tag with the EC50 of 33ng/ml determined by ELISA. ELISAData: Immobilized Biotinylated Human CD40, His Tag at 1g/ml (100l/well) on the plate. Dose response curve for Anti-CD40 Antibody, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA. Blocking DataSerial dilutions of Anti-CD40 Antibody were added into Biotinylated Human CD40, His Tag : Human CD40 Ligand (Trimer) , hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 2.0ug/ml. 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.