Recombinant Human CD27/TNFRSF7(hFc Tag)

Description :
FAQ COA 已发表文献 CD27, also known as TNFRSF7, is an approximately 55 kDa transmembrane protein in the TNF receptor superfamily. It functions as a co-stimulatory molecule that supports lymphocyte activation and survival. Mature human CD27 consists of a 172 amino acid (aa) extracellular domain (ECD) with three TNFR cysteine-rich repeats, a 21 aa transmembrane segment, and a 48 aa cytoplasmic domain. Within the ECD, human CD27 shares 63% and 66% aa sequence identity with mouse and rat CD27, respectively. CD27 is expressed as a disulfide-linked homodimer that carries N-linked and O-linked glycosylation. Proteolytic cleavage of CD27 results in the shedding of a 28-32 kDa fragment of the ECD. CD27 is weakly expressed on naive T cells and NK cells and is up-regulated upon cell activation. It is also up-regulated on activated germinal center B cells, plasma cells, and a subset of memory B cells. CD27 binds to the transmembrane glycoprotein CD27 Ligand/CD70 which is expressed on activated B cells, activated T cells, and dendritic cells. 产品性质 别名 CD27 antigen; Tp55; TNFRSF7; S152; T14; S152. LPFS2; MGC20393; S152CD27L receptor Uniprot No. P26842 Recombinant Human CD27/TNFRSF7Protein is expressed from HEK293Cellswith hFc tag at the C-terminal. It contains Thr21-Ile192. 分子量 Approximately 46.2 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. ELISAData: Immobilized Human CD27L Trimer at 2g/ml (100l/Well) on the plate. Dose response curve for Human CD27, hFc Tag with the EC50 of 0.2g/ml determined by ELISA. 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.