Biotinylated Recombinant Human PD-1/PDCD1 Protein, His-Avi Tag

Description :
FAQ COA 已发表文献 PD-1 a receptor expressed on T cells responsible for modulating T cell activation. PD-1 is classified as an immune checkpoint inhibitory receptor. PD1 is expressed on activated T cells, B cells, monocytes, and dendritic cells while PD-L1 expression is constitutive on the same cells and also on nonhematopoietic cells such as lung endothelial cells and hepatocytes. Ligation of PD-L1 with PD-1 induces co-inhibitory signals on T cells promoting their apoptosis, anergy, and functional exhaustion. 产品性质 别名 PDCD1; PD1; CD279; SLEB2; PD-1; Programmed Death-1 receptor Uniprot No. Q15116.1 Recombinant Biotinylated Human PD-1/PDCD1Protein is expressed from HEK293Cellswith His tag and Avi tag at the C-terminal. It contains Leu25-Gln167. 分子量 Approximately 18.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. ELISAData: Immobilized Biotinylated Human PD-1, His Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Anti-PD-1 Antibody, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA. SPR Data: Human PD-L1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human PD-1, His-Avi Tag with an affinity constant of 2.356 M as determined in SPR assay (Biacore T200). 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.