Recombinant Human DR3/TNFRSF25 (hFc Tag)

Description :
FAQ COA 已发表文献 Death receptor 3 (DR3), also known as lymphocyte-associated receptor of death (LARD), WSL-1, APO3, TRAMP and TR3, is a glycoprotein belonging to the TNF receptor superfamily (TNFRSF). DR3 was formerly designated TNFRSF12 when it was thought to be a receptor for TWEAK/TNFSF12. DR3 is one of six within the TNF R superfamily that contains a death domain in its cytooplasmic region. DR3 is expressed primarily in tissues enriched in lymphocytes. Whereas nave B and T cells express multiple truncated DR3 isoforms but not the transmembrane isoform 1, upon T cell activation, expression of the transmembrane DR3 isoform 1 predominates. TL1A/VEGI, a TNF superfamily ligand, has been shown to bind and activate DR3 (8). Depending on the cell context, ligation of DR3 by TL1A can trigger one of two signaling pathways. On primary T cells, TL1A induces NF-kappa-B activation and a costimulatory signal to increase IL-2 responsiveness and the secretion of proinflammatory cytokines. However, in a tumor cell line, TF-1, TL1A has been shown to induce caspase activity and apoptosis. In DR3-null mice, an impairment of negative selection and anti-CD3-mediated thymocyte apoptosis is observed. 产品性质 别名 APO3; DDR3; DR3; TNFRSF12; WSL; WSL1; TNFRSF25; LARD; TR3; TRAMP; WSL-LR Uniprot No. Q93038 Recombinant Human DR3/TNFRSF25Protein is expressed from HEK293Cellswith hFc tag at the C-terminal. It contains Gln25-Gln199. 分子量 Approximately 45.6 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.