Recombinant Human TIE2 Protein,His Tag

Description :
Synonyms Tie-2; Angiopoietin-1 receptor; TEK; VMCM; VMCM1; CD202b Source Human TIE2 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ala23-Leu748.[Accession | AAA61139.1] Molecular Weight The protein has a predicted MW of 82.09 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity SPR Data:Human TIE2, His Tag immobilized on CM5 Chip can bind Human ANGPT2, His Tag with an affinity constant of 2.38 M as determined in SPR assay . Formulation Lyophilized from 0.22 m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.