Recombinant Human LMP2 (HLA-A*02:01) Complex Tetramer Protein,His-Avi Tag

Description :
Source Recombinant Human LMP2 (HLA-A*02:01) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal,tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and CLGGLLTMV peptide.[Accession |A0A140T913(HLA-A*02:01)&P61769(B2M)&CLGGLLTMV] Molecular Weight The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity ELISA Data: Immobilized Human LMP2 (HLA-A*02:01) Tetramer, His Tag at 2g/ml (100l/Well) on the plate. Dose response curve for Anti-LMP2 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 9.6ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water