Recombinant Biotinylated Human GP100 (HLA-A*02:01) Complex Protein,His-Avi Tag

Description :
Source Recombinant Biotinylated Human GP100 (HLA-A*02:01) Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and YLEPGPVTA peptide.[Accession |A0A140T913(HLA-A*02:01)&P61769(B2M)&YLEPGPVTA] Molecular Weight The protein has a predicted MW of 50.4 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEand HPLC Activity ELISA Data:Immobilized Anti-GP100 (HLA-A*02:01) Antibody, hFc Tag at 2g/ml (100l/Well) on the plate. Dose response curve for Biotinylated Human GP100 (HLA-A*02:01) , His Tag with the EC50 of 0.88g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.