Recombinant Biotinylated Human HPV16 E7 (HLA-A*02:01) Protein,His-Avi Tag

Description :
Source Recombinant Biotinylated Human HPV16 E7(HLA-A*02:01) Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Gly25-Thr305(HLA-A*02:01) ,Ile21-Met119(B2M) and YMLDLQPET peptide.[Accession |P04439-1(HLA-A*02:01) &P61769(B2M)&YMLDLQPET peptide] Molecular Weight The protein has a predicted MW of 50.50 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGE > 95% as determined by HPLC Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.