Recombinant Human MAGE-A4 (HLA-A*02:01) Complex Tetramer Protein,His-Avi Tag

Description :
Source Recombinant Human MAGE-A4 (HLA-A*02:01) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal, tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and GVYDGREHTV peptide.[Accession |A0A140T913(HLA-A*02:01)&P61769(B2M)&GVYDGREHTV] Molecular Weight The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity ELISA Data: Immobilized Human MAGE-A4 (HLA-A*02:01) Tetramer, His Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Anti-MAGE-A4 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA. SPR Data: Anti-MAGE-A4 (HLA-A*02:01) Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Human MAGE-A4 (HLA-A*02:01) Tetramer, His Tag with an affinity constant of 8.49pM as determined in SPR assay Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.