Recombinant Human WT-1 (HLA-A*02:01) Complex Tetramer Protein,His-Avi Tag

Description :
Source Recombinant Human WT-1 (HLA-A*02:01) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal,tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and peptide.[Accession |A0A140T913(HLA-A*02:01)&P61769(B2M)&RMFPNAPYL] Molecular Weight The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity ELISA Data:Immobilized Human WT-1 (HLA-A*02:01) Tetramer, His Tag at 1g/ml (100l/Well) on the plate. Dose response curve for Anti-WT-1 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA. SPR Data: Anti-WT-1 (HLA-A*02:01) Antibody captured on Protein A chip, can bind Human WT-1 (HLA-A*02:01) Tetramer, His Tag with an affinity constant of 0.18nM as determined in a SPR assay Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.