Recombinant Human CD27 Ligand/CD70 Trimer Protein,His Tag

Description :
Source Recombinant Human CD27 Ligand/CD70 Trimer Protein is expressed from HEK293 with His tag at the N-terminal. It contains Leu50-Pro193.[Accession |P32970-1] Molecular Weight The protein has a predicted MW of 51.8 kDa. Due to glycosylation, the protein migrates to 62-65 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGE > 95% as determined by HPLC Activity ELISA Data: Immobilized Human CD27 Ligand Trimer, His Tag at 2g/ml (100l/Well) on the plate. Dose response curve for Human CD27, hFc Tag with the EC50 of 13.5ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.