Recombinant Biotinylated Human CD155/PVR Protein (Primary Amine Labeling),hFc Tag

Description :
Source Biotinylated Human CD155/PVR Protein (Primary Amine Labeling) is expressed from HEK293 with hFc tag at the C-terminal. It contains Trp21-Asn343.[Accession |P15151-1] Molecular Weight The protein has a predicted MW of 61.8 kDa. Due to glycosylation, the protein migrates to 75-82 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per ug by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity ELISA Data: Serial dilutions of Anti-TIGIT Antibody were added into Human TIGIT, His Tag : Biotinylated CD155, hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.46g/ml. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.