Recombinant Human/Cynomolgus/Rhesus macaque CD28 Protein,His-Avi Tag

Description :
Source Human/Cynomolgus/Rhesus macaque CD28 Protein is expressed from HEK293 with His tag and Avi tag at the N-terminal. It contains Asn19-Pro152.[Accession |P10747-1] Molecular Weight The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 36-52 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity ELISA Data: Immobilized Human/Cynomolgus/Rhesus macaque CD28, His Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 of 15.7ng/ml determined by ELISA. SPR Data: Anti-CD28 antibody captured on Protein A chip, can bind Human/Cynomolgus/ Rhesus macaque CD28, His Tag with an affinity constant of 5.15nM as determined in a SPR assay. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.