Recombinant Biotinylated Human CD40 Ligand/TNFSF5 Trimer Protein (Primary Amine Labeling) ,His-Flag Tag

Description :
Source Biotinylated Human CD40 Ligand/TNFSF5 Trimer Protein (Primary Amine Labeling) is expressed from HEK293 with His tag and Flag tag at the N-terminal. It contains Met113-Leu261.[Accession |P29965] Molecular Weight The protein has a predicted MW of 52.4 kDa, Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity ELISA Data: Immobilized Biotinylated Human CD40 Ligand Trimer, His Tag at 2g/ml (100l/well) on the streptavidin precoated plate (5g/ml). Dose response curve for Human CD40, hFc Tag with the EC50 of 0.61g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.