Recombinant Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein,hFc Tag

Description :
Source Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein is expressed from HEK293 with hFc tag at the C-terminal. It containsGln22-Asp117(CD3E)&Gln23-Thr113(CD3G). [Accession |Q95LI5.2(CD3E)&Q95LI7(CD3G)] Molecular Weight The protein has a predicted MW of 36.9 kDa (CD3E) and 36.5 kDa (CD3G). Due to glycosylation,the protein migrates to 44-46 kDa&46-48 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity ELISA Data:Immobilized Cynomolgus CD3E&CD3G, hFc Tag at 0.5g/ml (100l/Well). Dose response curve for Biotinylated Anti-CD3 Antibody, mFc Tag with EC50 of 51.2ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.