Recombinant Cynomolgus CD27/TNFRSF7 Protein,His Tag

Description :
Source Cynomolgus CD27/TNFRSF7 Protein is expressed from HEK293 with His tag at the C-terminal. It contains Thr21-Ile192.[Accession |XP_005569963.1] Molecular Weight The protein has a predicted MW of 20.41 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGE > 95% as determined by HPLC Activity ELISA Data:Immobilized Cynomolgus CD27, His Tag at 1g/ml (100l/well) on the plate. Dose response curve for Human CD27 Ligand, hFc Tag with the EC50 of 0.23g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.