Recombinant Biotinylated Human Siglec-10 Protein,His-Avi Tag

Description :
Source Biotinylated Human Siglec-10 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Met17-Thr546.[Accession |Q96LC7-1] Molecular Weight The protein has a predicted MW of 61.13 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEand HPLC Activity ELISA Data: Immobilized Anti-Siglec-10 Antibody, hFc Tag at 2g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human Siglec-10, His Tag with the EC50 of 0.25g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in 20mM Tris, 150mM Nacl, 200mM Arginine (pH 8.5). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.