Recombinant Biotinylated Human VEGF121 Protein,His-Avi Tag

Description :
Source Biotinylated Human VEGF121 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Ala27-Arg147.[Accession |P15692-9] Molecular Weight The protein has a predicted MW of 17 kDa. Due to glycosylation, the protein migrates to 18 kDa and 22-25 kDa under reduced (R) condition, 30 kDa and 32-40 kDa under Non reducing (N) condition based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGE Activity ELISA Data: Immobilized Human VEGF R1, His Tag at 2g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human VEGF121, His Tag with the EC50 of 36.5ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.