Recombinant Human IL-15RA&IL-15 Protein,His Tag

Description :
Source Human IL-15RA&IL-15 Protein is expressed from HEK293 with His tag at the C-terminal. It contains Ile31-Ser108(IL-15RA)&Asn49-Ser162(IL-15).[Accession |Q13261-1&P40933-1] Molecular Weight The protein has a predicted MW of 23.8 kDa. Due to glycosylation, the protein migrates to 26-28 kDa&30-32 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per ug by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity ELISA Data: Immobilized Human IL-15RA&IL-15, His Tag at 1g/ml (100l/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC50 of 72.0ng/ml determined by ELISA SPR Data: Human IL-2 R beta&IL-2 R gamma, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-15Ra&IL-15, His Tag with an affinity constant of 0.084 nM as determined in SPR assay Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.