Recombinant Human LDLR Protein,His-Avi Tag

Description :
Source Human LDLR Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Ala22-Arg788.[Accession |P01130-1] Molecular Weight The protein has a predicted MW of 87.6 kDa, Due to glycosylation, the protein migrates to 110-130 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGE Activity SPR Data: Human LDLR, His Tag immobilized on CM5 Chip can bind Human PCSK9, His Tag with an affinity constant of 0.35 nM as determined in SPR assay Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.