Rabbit anti-Nephrin Polyclonal Antibody

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Product Details FAQ Citations(0) Video Pictures Documents |Overview |Synonym CNF; NPHN; Nephrosis 1 congenital Finnish type; NPHS 1; NPHS1; Renal glomerulus specific cell adhesion receptor; Renal glomerulus-specific cell adhesion receptor; NPHN_HUMAN |Host Rabbit |Specificity Nephrin |Species Reactivity Human, Mouse, Rat, Dog |Predicted Reactivity Rabbit |Applications WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 Flow-Cyt=1μg/Test |Immunogen KLH conjugated synthetic peptide derived from human Nephrin:451-550/1241 |Properties |Concentration 1mg/ml |Purification affinity purified by Protein A |Clonality Polyclonal Antibody |Isotype IgG |Storage Temp. Store at -20 ° C for one yearAvoid repeated freeze/that cycles |Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |Target |Background This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.[provided by RefSeq, Oct 2009] |Cellular localization Extracellular matrix; |UniProt O60500 | Sample: MDCK(Dog) Cell Lysate at 40 ugPrimary: Anti-Nephrin (abs120474) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 138 kDObserved band size: 138 kD| Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-Nephrin Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining| Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-Nephrin Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining| Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.Isotype Control Antibody: Rabbit IgG ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).| the cells(293T) were fixed with 2% paraformaldehyde (10 min).Isotype Control Antibody: Rabbit IgG ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green). |Tips:This product is for research use only. Not for use in diagnostic prodcedures.