Recombinant Human Clusterin Protein,His Tag

Description :
Source Human Clusterin Protein is expressed from HEK293 with His tag at the C-terminal. It contains Asp23-Glu449.[Accession |P10909-1] Molecular Weight The protein has a predicted MW of 51.2 kDa. The protein migrates to 38-41 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per ug by the LAL method. Purity > 95% as determined by Tris-Bis PAGE Activity ELISA Data: Immobilized Human Clusterin, His Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Anti-Clusterin Antibody, hFc Tag with the EC50 of 49.2ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.