Recombinant Human NGAL/Lipocalin-2 Protein,hFc Tag

Description :
Source Human NGAL/Lipocalin-2 Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Gln21-Gly198.[Accession |P80188-1] Molecular Weight The protein has a predicted MW of 47.2 kDa. Due to glycosylation, the protein migrates to 48-52 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity ELISA Data: Immobilized Human NGAL, hFc Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Biotinylated Anti-NGAL Antibody, hFc Tag with the EC50 19.7ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in 50mM MES,150mM NaCl (pH 6.5). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in 50mM MES,150mM NaCl (pH 6.5).