Recombinant Human SPARC Protein,His Tag

Description :
Source Human SPARC Protein is expressed from HEK293 with His tag at the C-terminal. It contains Ala18-Ile303.[Accession |P09486] Molecular Weight The protein has a predicted MW of 33.8 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity ELISA Data: Immobilized Human SPARC, His Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Anti-SPARC Antibody, hFc Tag with the EC50 of 0.10g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.