Recombinant Human IL-23R Protein,His Tag

Description :
Source Human IL-23R Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gly24-Gly355.[Accession |Q5VWK5-1] Molecular Weight The protein has a predicted MW of 39.15 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity ELISA Data: Immobilized Human IL-23R, His Tag at 5g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human IL-23 alpha&IL-12 beta, His Tag with the EC50 of 0.87g/ml determined by ELISA SPR Data: Human IL-23R, His Tag immobilized on CM5 Chip can bind Human IL-23 alpha&IL-12 beta, His Tag with an affinity constant of 5.72 nM as determined in SPR assay (Biacore T200). Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.