Recombinant Human IL-23R Protein,mFc Tag

Description :
Source Human IL-23R Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus. It contains Gly24-Gly355.[Accession |Q5VWK5-1] Molecular Weight The protein has a predicted MW of 63.7 kDa. Due to glycosylation, the protein migrates to 80-115 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin 1.0 EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEand HPLC Activity ELISA Data: Immobilized Human IL-23 alpha&Mouse IL-12, His Tag at 5g/ml (100l/Well) on the plate. Dose response curve for Human IL-23R, mFc Tag with the EC50 of 0.88g/ml determined by ELISA SPR Data: Human IL-23R, mFc Tag captured on CM5 Chip via Anti-Mouse Antibody can bind Human IL-23 alpha&IL-12 beta, His Tag with an affinity constant of 11.09 nM as determined in SPR assay (Biacore T200). Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.