Recombinant Human/Murine/Rat Activin A Protein

Description :
Synonyms Activin A, Activin AB alpha polypeptide, inhibin beta A chain Uniprot No. P08476 Source Human/Murine/Rat Activin AProteinis expressed from CHO cell.It contains Gly311 – Ser426. Molecular Weight Apparent molecular mass of 24 kDa in SDS-PAGE under non-reducing conditions, 14 kDa under reducing conditions, a disulfide-linked homodimer of two 116 amino acid glycosylated polypeptide chains. Biological Activity Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. The ED50for this effect is 0.2-1.2 ng/mL.The specific activity of Activin A is approximately 1 IU/g. Purity > 95% as determined by SDS-PAGE. Endotoxin Formulation Lyophilized from 0.2 m filtered concentrated solution in 30 % acetonitrile and 0.1 % TFA. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile 4 mM HCl to a concentration of 0.1-0.5 mg/ml. Further dilutions should be made in appropriately buffered solutions.