Recombinant Human PLAU/uPA Protein (pro form) ,His Tag

Description :
Source Human PLAU/uPA Protein (pro form) is expressed from HEK293 with His tag at the C-Terminus. It contains Ser21-Leu431.[Accession |P00749-1] Molecular Weight The protein has a predicted MW of 47.5 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin 1EU per g by the LAL method. Purity > 95% as determined byTris-Bis PAGEandHPLC. Activity ELISA Data: Immobilized Human PLAU, His Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Human uPAR, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in 25mM HEPES, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.