Recombinant Human PCSK9 Protein,His Tag

Description :
Source Recombinant Human PCSK9 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln31-Gln692.[Accession |Q8NBP7-1] Molecular Weight Due to autocatalytic cleavage, the protein release the pro-form (59 kDa) and mature form (14 kDa). Due to glycosylation, the protein migrates to 65-68 kDa (pro-form) and 15kDa (mature form) based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity ELISA Data:Immobilized Human PCSK9, His Tag at 2g/ml (100l/Well) on the plate. Dose response curve for Anti-PCSK9 antibody, hFc Tag with the EC50 of 0.16g/ml determined by ELISA . SPR Data:Human LDLR, His Tag immobilized on CM5 Chip can bind Human PCSK9, His Tag with an affinity constant of 0.35 nM as determined in SPR assay (Biacore T200). Formulation Supplied as 0.22m filtered solution in 20mM PB, 300mM NaCl, 10% Glycerol (pH 7.4).